We can verify that this ordered mechanism is not random by demonstrating that no B (ethanol) is bound to E in the absence of A (NAD+).
Double-Displacement (Ping-Pong) Reactions
Reactions conforming to this kinetic pattern are characterized by the fact that the product of the enzyme's reaction with A (called P in the following schemes) is released prior to reaction of the enzyme with the second substrate, B. As a result of this process, the enzyme, E, is converted to a modified form, E', which then reacts with B to give the second product, Q and regenerate the unmodified enzyme form, E:
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