Equipment

1. 15-mL conical tubes.

2. Sterile, autoclavable scissors and forceps (2 pairs of each).

3. Sterile, autoclavable glass homogenizer (7 mL for up to two spleens, 2 mL for one spleen or lymph nodes, LNs).

4. Hemocytometer and coverslip.

5. Microcentrifuge tubes for counting cells.

8. Sterile 1-mL syringes and 26-ga needles.

9. 162, 75, 25 cm2 tissue culture flasks.

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