Renal Disease Assessment

2.3.1. Light Microscopy

1. Acid/alcohol differentiator: 10% acetic acid in 95% ethanol.

2. Coverslips for microscope slides.

3. Eosin Y Alcoholic (e.g., EM Science, Gibbstown, NJ, 588X-75).

5. Mercury-free Harris Hematoxylin (e.g., EM Science 638A-71).

6. Poly-L-lysine-coated microscope slides.

7. Mounting media.

8. Paraffin.

9. Distilled or deionized and tap water. 10. Xylene.

2.3.2. Immunofluorescence for Immune Deposits

2. Coverslips for microscope slides.

3. Cryostat.

5. Fluorescence-ready microscope.

6. Poly-L-lysine-coated microscope slides.

7. Mounting media.

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