CENP-F has several features that make it an attractive candidate for a marker of cell proliferation in tumors. First, its expression is proliferation-dependent and relatively specific for the G2 and M phases of the cell cycle. Second, its cell cycle-dependent distribution is highly conserved in mammalian cell lines [24]. Third, its association with the nuclear matrix confers resistance to a wide variety of preparation and fixation procedures [24, 31]. Three studies have been conducted to determine the potential use of CENP-F as a proliferation marker. The first study used CENP-F autoantibodies in two-parameter flow cytometry to correlate CENP-F expression with S-phase fraction in 24 hematological tumors, 12 breast cancers, and several cultured cell lines [31]. A significant correlation was observed between the percentage of CENP-F positive cells and S-phase fraction for all the tumors analyzed [31], In the second study, an anti-mitosin monoclonal antibody, designated 14C10, was used in immunohis-tochemistry to evaluate mitosin/CENP-F expression in 386 node-negative, formalin-fixed, archival breast cancers [32], A strong positive correlation was found in this study between CENP-F expression and S-phase fraction. The expression of the protein correlated negatively with other prognostic factors such as estrogen

Table 1. Clinical features of patients with CENP-F antibodies

Patient No.



Clinical feature

10 Ways To Fight Off Cancer

10 Ways To Fight Off Cancer

Learning About 10 Ways Fight Off Cancer Can Have Amazing Benefits For Your Life The Best Tips On How To Keep This Killer At Bay Discovering that you or a loved one has cancer can be utterly terrifying. All the same, once you comprehend the causes of cancer and learn how to reverse those causes, you or your loved one may have more than a fighting chance of beating out cancer.

Get My Free Ebook

Post a comment