Conclusion

Our institution is a 200-bed pediatric hospital. We offer both Hb variant and Hb A1c analyses three days a week throughout the year. With our present procedure, sample to sample run time is approx 18 min including all rinses and injections, or about 3 samples/h. With a P/ACE 2200 (Beckman Instruments, Fullerton, CA) samples can run unattended during the day or overnight, with a maximum of 20 samples (~7 h) loaded on the autosampler at one time. This means about 40 samples can be analyzed in one working day. This number of samples would require 2-3 technologist hours per day for instrument setup, data reduction and reporting, and routine administrative responsibilities.

Annually, we perform approx 400 tests to diagnose or monitor congenital hemoglobinopathies, especially sickle-cell disease, and over 1000 tests for Hb A1c to manage diabetic patients. Reagents and consumables, including capillaries, cost only a few cents per test. Controls are inexpensively prepared in our laboratory from RBC and can be stored at -70°C (not at -20°C) in hemo-lyzing reagent for at least 2 yr. Between-run imprecision (% CV) for a normal control containing 2.7% Hb A2 and 97.3% Hb A was 3.5 and 0.1%, respectively, based on 50 replicate analyses performed on different days over a 5-mo period with multiple capillaries (8). Between run imprecision for controls containing 8.3 and 15.6% Hb A1c was 2.3 and 2.0% CV, respectively, in 40 replicate analyses performed on different days over a 5-mo period.

Given the advantages of capillary electrophoresis for the analysis of hemoglobin variants, including both structural and posttranslational variants, it is surprising that a major manufacturer of analytical instrumentation has not yet had the foresight to develop a commercially available system for these applications.

Diabetes 2

Diabetes 2

Diabetes is a disease that affects the way your body uses food. Normally, your body converts sugars, starches and other foods into a form of sugar called glucose. Your body uses glucose for fuel. The cells receive the glucose through the bloodstream. They then use insulin a hormone made by the pancreas to absorb the glucose, convert it into energy, and either use it or store it for later use. Learn more...

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