for both CA II and CA IV, and, more importantly, were unable to cross the plasma membranes in vivo (Supuran et al. 2000b; Scozzafava et al. 2000c). In two model systems (human red cells and perfusion experiments in rats), this new class of potent, positively charged CAIs was able to discriminate the membrane-bound from the cytosolic isozymes, selectively inhibiting CA IV only (Supuran et al. 2000b; Scozzafava et al. 2000c). Such data are important both for specific in vivo inhibition of membrane-associated isozymes and for the eventual development of some novel anticancer therapies, because it has been shown that some tumor cells predominantly express only some membrane-associated CA isozymes, such as CA IX and CA XII.
This type of selective CAI might be of great relevance to different physiological studies. For example, Sterling et al. (2002) investigated the functional and physical
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