Index

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Contributors

Work Plan Tissue Culture

Barbara Clough

National Institute for Medical Research, Mill Hill, London NW7 IAA, UK Section: 1.2

Martin Clynes

National Cell and Tissue Culture Centre, Bioscience Ireland, School of Biological Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland Section: 2.6

Thomas G. Cotter

Department of Biochemistry, University College Cork, Lee Makings, Prospect Row, Cork, Ireland Section: 4.4

Irene Cour

American Type Culture Collection, Cell Culture Department, PO Box 1549, Manassas, Virginia 20108 1549, USA Section: 1.8

Margaret Dooley

National Cell and Tissue Culture Centre, Bioscience Ireland,

School of Biological Sciences, Dublin City University, Glasnevin, Dublin 9,

Ireland

Section: 2.6

Alan Doyle

Scientific Programme Manager, Joint Infrastructure Fund, The Wellcome Trust, 183 Euston Road, London NW1 2BE, UK Sections: 1.1, 1.3, 1.4, 1.6, 3.1, 3.6 & 6.1

Jean-Marc Engasser

Institut National Polytechnique de Lorraine, Laboratoire des Sciences du Genie Chimique, CNRS-ENSIC-ENSAIA, BP 451, 1 Rue Grandville, 54001 Nancy Cedex, France Section: 4.3

Jean-Louis Goergen

Institut National Polytechnique de Lorraine, Laboratoire des Sciences du Genie Chimique, CNRS-ENSIC-ENSAIA, BP 451, 1 Rue Grandville, 54001 Nancy Cedex, France Section: 4.3

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Angela Martin

National Cell and Tissue Culture Centre, Bioscience Ireland, School of Biological Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland Section: 2.6

Jennie P. Mather

Genentech Inc., 460 San Bruno Boulevard, San Francisco, CA 94080, USA Section: 3.2

Mary Mazur-Melnick

Connaught Labs Ltd, 1775 Steeles Avenue West, Willowdale, Ontario M24 3T4,

Canada

Section: 5.7

William M. Miller

Northwestern University, Robert R.McCormick School of Engineering and Applied Science, Department of Chemical Engineering, 2145 Sheridan Road, Evanston, IL 60208-3120, USA Section: 4.2

Jon Mowles

Biochem Immuno Systems UK Ltd, 20 Woking Business Park, Albert Drive, Woking GU21 5JY, UK Sections: 1.6 & 1.9

T. Ohno

Riken Gene Bank, 3-1-1 Koyadoi, Yatabe, Tsukuba, Ibaraki 305, Japan Section: 1.6

Eleftherios T. Papoutsakis

Northwestern University, Department of Chemical Engineering, 2145 Sheridan Road, Evanston, IL 60208, USA Sections: 4.6, 4.7 & 4.8

Andrew Racher

LONZA Biologies pic, 228 Bath Road, Slough, Berkshire SL1 4DY, UK Section: 2.5

Sridhar Reddy

Associate Scientist, Cell Genesys, Inc., 322 Lakeside Dr., Foster City, CA 94404 USA

Section: 4.2 0ystein R0nning

Optoflow AS, Olaf Helsets Vei 6, N-0621 Oslo, Norway Section: 4.5

Winfried Scheirer

Novartis Forschungsininstitut GmbH, Cellular/Molecular Biology, PO Box 80, Branner Strasse 59, A-1235 Vienna, Austria Section: 5.10

Ulrich Sehuerch

Swiss Serum and Vaccine Institute, CH 3001, Berne, Switzerland Sections: 5.4 & 5.5

Glyn Stacey

NIBSC, Blanche Lane, South Mimms, Potters Bar EN6 3QG, UK Sections: 1.3, 1.5, 3.7 & 6.1

R. Teyssou

Université de Bordeaux II, Laboratoire de Bactériologie, 146 rue Leo Saignat, 33076 France Section: 1.7

Mary C. Tsao

Genentech Inc., 460 San Bruno Boulevard, San Francisco, CA 94080, USA Section: 3.3

Sally Warburton

ECACC, CAMR, Salisbury, Wiltshire SP4 0JG, UK Section: 2.2

Kristina Zachrisson

Pharmacia and Upjohn AB, Strandbergsgatan 47, S-112-87 Stockholm, Sweden Section: 3.4

Foreword

Preface

Safety

Neither the editors, contributors nor John Wiley & Sons Ltd. accept any responsibility or liability for loss or damage occasioned to any person or property through using the materials, instructions, methods, or ideas contained herein, or acting or refraining from acting as a result of such use. While the editors, contributors and publisher believe that the data, recipes, practical procedures and other information, as set forth in this book, are in accord with current recommendations and practice at the time of publication, they accept no legal responsibility for any errors or omissions, and make no warranty, express or implied, with respect to material contained herein. Attention to safety aspects is an integral part of all laboratory procedures and national legislations impose legal requirements on those persons planning or carrying out such procedures. It remains the responsibility of the reader to ensure that the procedures which are followed are carried out in a safe manner and that all necessary safety instructions and national regulations are implemented.

In view of ongoing research, equipment modifications and changes in governmental regulations, the reader is urged to review and evaluate the information provided by the manufacturer, for each reagent, piece of equipment or device, for any changes in the instructions or usage and for added warnings and precautions.

CHAPTER 1

THE CELL: SELECTION AND STANDARDIZATION

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1.1 OVERVIEW

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BIOTECHNOLOGISTS

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