Design of Hollow Fibre Modules

Initially we developed a small-scale parallel-plate system to study the influence of shear stress on separation performance 9 , but larger surface areas could be manufactured cost-effectively using the hollow fibre spinning technology developed for renal dialysis 3,4 . A typical renal dialyser is a hollow fibre array housed within a polycarbonate shell with two independent flow paths (intra- and extra-capillary) separated by a dialysis membrane. The intra-capillary surface area is approximately...

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Separation of bioparticles by centrifugation involves the use of a density gradient medium, a reagent solution that forms a density gradient over the desired range. In this method, pH and osmolality are easily adjustable allowing preservation of the integrity of cells. The sample for example blood is layered on the medium and centrifuged to separate low-density from high-density cells. Several of the separation methods to be discussed rely on density gradient pre-enrichment techniques. Widely...

Detachment of Bound Particles by Elastic Deformation

Cryogel Liquid

Adsorption of bioparticles, especially as big as microbial cells, to affinity surfaces involves polyvalent interactions complicating greatly the recovery of the adsorbed material. Different strategies have been tested in an attempt to overcome the problem of strong multipoint attachment of cells to affinity surfaces. For example, in a membrane-based chromatographic system bound B-cells were released by transmembrane diffusion of hydrochloric acid pH 1 into a flow of neutralizing normal saline...

References

Ugelstad J, M0rk PC, Herder Kaggerud K, Ellingsen T, Berge A 1980 Swelling of oligomer particles New methods of preparation of emulsions and polymer dispersions. Adv Colloid Interface Sci 13 101 2. Ugelstad J, Kilaas L, Aune O, Bj0rgum J, Herje R, Schmid R, Stenstad P, Berge A 1994 Monodisperse polymer particles. In Uhlen M, Hornes E, Olsvik 0 eds Advances of Biomagnetic Separation. Eaton Publ Comp, Natick, MA, pp 1-20 3. Funderud S, Nustad K, Lea T, Vartdal F, Gaudernack G, Stenstad P,...