Assessment Of Hematopoietic Chimerism

The assessment of hematopoietic chimerism required more sensitive techniques than conventional cytoge-netic analyses because of the availability of only small numbers of dividing cells. The most widely used techniques have been fluorescent in situ hybridization (FISH), with X- and Y-specific probes in case of sex-

mismatched transplant, and polymerase-chain-reaction-based assays of polymorphic mini- (variable number tandem repeats) or micro-satellite markers in case of sex-matched transplant. Other techniques based on restriction fragment length polymorphism have also been used.

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