Immunohistochemistry

Several B-cell associated antibodies, including CD20, CD79a, and DBA.44, can be used in routinely processed paraffin tissue sections of the bone marrow to detect hairy cells, Figure 30.4. Although these antibodies are not specific for HCL, they can be used to document the

Figure 30.4 Immunohistochemistry stain (MB2) of bone marrow trephine biopsy indicating that hairy cells are of B-cell origin

B-cell nature of the infiltrate and highlight the extent of bone marrow infiltration at the time of diagnosis and following therapy.5657 Immunohistochemical techniques can also be used to evaluate bone marrow sections for TRAP.42

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