Chromatin Reconstitution on Immobilized DNA

Chromatin Reconstitution Using Drosophila Embryo Extracts 1. Resuspend stock of immobilized template. Pipet out appropriate amount of bead-DNA. 900 ng of DNA is sufficient for a MNase assay or analysis of bound his-tone by silver staining. 2. Concentrate on the MPC. Remove supernatant and wash once with 300 L of PBS-BSA-NP40. 3. Wash again with 300 L EX-NP40. 4. Prepare chromatin assembly reaction by mixing 70 L chromatin assembly extract, 12 L McNAP, and 38 L EX buffer for each 900 ng...