Info

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Note: +/— represents high/low concentration of the component.

Note: +/— represents high/low concentration of the component.

medium was optimized for human interferon-gamma production (132). A series of components, including many amino acids, BSA, and other growth factors, were studied. From this, glycine was discovered to affect the specific growth rate, as well as BSA, phenylalanine, and tyrosine. Other amino acids, such as methionine, proline, and histidine played an important role in interferon-gamma production. On the other hand, insulin, arginine, aspartate, and serine produced an inhibitory effect on both cell growth and interferon-gamma production. The end result was an optimized medium that yielded 45% higher productivity than previous formulations.

Similarly, using the same statistical approach, an improved medium for the production of erythropoietin from CHO cells was developed (144). This medium was based on an IMDM basal medium, supplemented with Fe(NO3)3 9H2O, CuCl2,ZnSO4.7H2O, insulin, transferrin, and ethanolamine. Glutamate, serine, methionine, phosphatidylcholine, hydrocortisone, and pluronic F68 were identified as positive determinants for cell growth, and the final optimized medium yielded 79% higher productivities than serum-supplemented IMDM.

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