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103 104 10s 106 107 Cell Concentration (c/ml)

103 104 10s 106 107 Cell Concentration (c/ml)

Figure 7 Correlation of fluorescence and number of cells per well for CHO cells grown in 96-well tissue culture plates. Fluorescence was measured in a 96-well plate reader after various lengths of exposure to calcein-AM.

affordable. mRNA screens may not be useful in extreme cases where protein secretion is generally limited by factors other than transcription, but for most applications, cells that produce high quantities of product mRNA are good targets to move into more complex screening paradigms.

Generally two to three rounds of microtiter scale screening are employed to segregate the top 1-5% of clones that will be expanded for further evaluation.

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