Ultrasonic bath (output. 150 W; frequency. 35 KHz); samples sieved (2 mm) and stored at room temperature until fortified; soil samples were extracted with 4 ml of ethyl acetate for 15 min in an ultrasonic water bath at room temperature; the water level in the bath was adjusted to equal the extraction solvent level inside the columns; soil samples were extracted again with another 4 ml of ethyl acetate (15 min); total extract. 2 to 5 ml (stored at 4°C); solvent, methanol or ethyl acetate
175°C with dichloromethane-acetone (1:1, v/v) with 8 min heat-up time and 5 min static time
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