Table 119

Analysis of Amines by HPLC

E: 5 mM phosphate buffer, pH 3-acetonitrile-water (90:10)

C: 150 X 4.6 mm (Prodigy ODS RP-18, 5 ju,m) E: methanol-acetonitrile-buffer phosphate 0.01 M (30:30:40) F: 1 ml/min

C: 250 X 4.6 mm (Chrompack Inertsil ODS-2) E: methanol-water-buffer pH 3.40 (60:35:5) F: 0.2 ml/min

C: 100 X 4.6 mm (Partisil 5 ju,m C18) E: methanol-water (45:55) C: 150 X 3.9 mm (Nova-Pak 4 ju,m C18) E: acetonitrile-acetate buffer pH 4.66 (40:60) F: 0.2 ml/min

C: 250 X 4.6 mm (I.D. ODS-2.5 ju,m) E: acetonitrile-water (30:70) F: 1 ml/min

C: 250 X 4.6 mm (10 jum Spherisorb Nitric and Hexyl) E: methanol-acetonitrile F: 1 ml/min

C: 250 X 4.6 mm (10 |j,m Lichrosorb RP-C8) E: water-ammonium acetate buffer and acetonitrile-water (80:20) F: 0.8 ml/min, 40°C

C: 150 X 3.9 mm (Nova-Pak C18) E: acetonitrile-acetate buffer pH 4.66 (40:60) F: 1 ml/min

C: 250 X 4.6 mm (ODS 80T, 5 ju,m) E: ammonium acetate pH 6.0-acetonitrile (90:10), ammonium acetate pH 6.0-acetonitrile (70:30) C: 250 X 4.6 mm (I.D. 5 ju,m C18) E: methanol-water (46:54) and buffer pH 5.40 F: 1 ml/min

C: 250 X 4 mm (Spherisorb ODS-2, 10 ju,m C18) E: acetate buffer pH 4.0-acetonitrile (30:70) F: 1 ml/min

E: various ratios of KNO3, NaH2PO4, KH2PO4, H2O, 85% H3PO4, acetonitrile F: 1 or 2 ml/min

C: 2.6 X 50 mm cation exchange resin

E: 0.045 M sodium citrate, 0.061 M citric acid, 0.064 M NaCl and 0.20 M sodium citrate,

2 M NaCl, pH 7.0 C: 100 X 3 mm (Genesis C18; I.D. 4 ju,m)

E: methanol-10 mM ammonium formate, pH 4 in water-methanol (80:20) F: 0.3 ml/min

Reference

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