A

Figure 2.7. Demonstration of a p-lactamase reporter cell line. In a final volume of 0.02 mL, 5000-10,000 cells were seeded overnight at 37°C before being stimulated with excess agonist or antagonist for 4 h. The resultant [3-lactamase activity was measured after a 1-h incubation with CCF2 (Aurora Biosciences) at the indicated wavelengths in an LJL Analyst fluorometer. (a) The unstimulated cells, (b) the cells incubated with agonist, and (c) cells treated with agonist and excess antagonist.

Figure 2.7. Demonstration of a p-lactamase reporter cell line. In a final volume of 0.02 mL, 5000-10,000 cells were seeded overnight at 37°C before being stimulated with excess agonist or antagonist for 4 h. The resultant [3-lactamase activity was measured after a 1-h incubation with CCF2 (Aurora Biosciences) at the indicated wavelengths in an LJL Analyst fluorometer. (a) The unstimulated cells, (b) the cells incubated with agonist, and (c) cells treated with agonist and excess antagonist.

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