Notes

This procedure will take approximately

© Dissolve 1.33 g lead citrate in 30 ml distilled water and add 1.76 g sodium citrate. Mix the resulting suspension for 1 min and allow to stand for 30 min. Add 8.0 ml 1 N NaOH and make up to 50 ml with distilled water and mix. Store the solution in a well-sealed dark bottle at 4 °C and centrifuge/filter before use [14].

(2 Omit steps 1 -3 if the specimen material has been block stained with uranyl acetate.

(3 Sections may be mounted on formvar-coated EM grids, for greater stability.

(4 Use NaOH washing if lead citrate staining produces precipitate on the sections.

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