Preparation and staining of specimens


When working with tissue culture cells or cell smears, the cells to be stained need to


be attached to a solid support, usually a microscope slide or coverslip, to facilitate handling. This can be done in a number of ways. Adherent cells can be grown on coverslips in Petri dishes or in special slide chambers. Poorly adherent cells can be encouraged to attach to glass or plastic in culture by precoating the coverslips or slides with 1% (w/v) gelatin in distilled water or 500 ^g/ml poly-L-lysine in distilled water. For easier handling suspension cells can be bound to slides using chemical linkers or by centrifuging onto slides using a cytocentrifuge [3].

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